‘DD’ as a Means of Controlling Heterodera rostochiensis (Woll.)

The chemical composition of the egg shells of the potato cyst-nematode, Heterodera rostochiensis Woll.

1. Eggs of the potato cyst-nematode (Heterodera rostochiensis Woll.) were isolated by sieving a suspension of crushed cysts. Eggs were broken open by ultrasonic vibration and the egg shells separated from the released larvae by centrifuging in a potassium tartrate density gradient. About 1 mg. of dried egg shells was obtained from 1000 cysts. 2. The major constituent of the egg shells was prote...

Molecular Characterization of Aldolase from Heterodera glycines and Globodera rostochiensis.

Fructose-bisphosphate aldolase (EC 4.1.2.13) is a key enzyme in glycolysis. We have characterized full-length coding sequences for aldolase genes from the cyst nematodes Heterodera glycines and Globodera rostochiensis, the first for any plant-parasitic nematode. Nucleotide homology is high (83% identity), and the respective sequences encode 40 kDa proteins with 89% amino acid identity. Genomic ...

Zoning as a Means of Controlling Commercial Strip Development

The chemical composition of the cyst wall of the potato cyst-nematode, Heterodera rostochiensis.

1. Cyst walls of the potato cyst-nematode (Heterodera rostochiensis Woll.) were isolated by sieving a suspension of crushed cysts. About 12mg. of dried cyst walls was obtained from 1000 cysts. 2. The cyst walls contained mainly protein (72%, calculated from nitrogen content). On acid hydrolysis about 77% of the cyst wall went into solution. Of 19 amino acids present, proline, glycine, and alani...

Electrical stimulation applied to second-stage larvae of Heterodera rostochiensis to determine viability.

Viability of cysts of the golden nematode, Heterodera rostochiensis Wollenweber, recovered f rom treated fields on Long Island, New York, is based on appearance and movement of second-stage larvae. If internal structures of one or more larvae appear to be normal in appearance, observations are made at r andom intervals for 1-2 days to detect movement . Th is is titheconsuming and quest ionable ...